An agroindustry water discharge sample was taken to the laboratory An agroindustry water discharge sample was taken to the laboratory to analyse alkanes and polyaromatic hydrocarbons (PAHs) with a wide range of polarity. The compounds can be analysed using three separation methods: gas chromatography (GC), high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC).i) Explain why the faster analysis was achieved using SFC with supercritical fluid CO2 as a mobile phase compared to HPLC with methanol:water as a mobile phase. (3 marks)ii) The affinity of the analytes towards the mobile phase in SFC, is said to be a function of density. Explain the meaning of this statement. (2 marks)iii) Write an order of B term (molecular diffusion) and C term (mass transfer) on band broadening (H) for SFC, HPLC and GC systems. (2 marks)iv) Separation by SFC resulted in all alkanes being eluted in less than 2 minutes. However, PAHs were eluted in the range of 5-7 minutes. Discuss a way to reduce the analysis time of this separation. (4 marks)v) SFC using carbon dioxide (CO2) as the mobile phase and silica as the stationary phase is generally equated to a normal phase partition High Performance Liquid Chromatography (HPLC). Explain this statementScience Chemistry
